A Human 3′ miR-499 Mutation Alters Cardiac mRNA Targeting and Function
نویسندگان
چکیده
منابع مشابه
A human 3' miR-499 mutation alters cardiac mRNA targeting and function.
RATIONALE MyomiRs miR-499, miR-208a and miR-208b direct cardiac myosin gene expression. Sequence complementarity between miRs and their mRNA targets determines miR effects, but the functional consequences of human myomiR sequence variants are unknown. OBJECTIVE To identify and investigate mutations in human myomiRs in order to better understand how and to what extent naturally-occurring seque...
متن کاملElevated miR-499 Levels Blunt the Cardiac Stress Response
BACKGROUND The heart responds to myriad stresses by well-described transcriptional responses that involve long-term changes in gene expression as well as more immediate, transient adaptations. MicroRNAs quantitatively regulate mRNAs and thus may affect the cardiac transcriptional output and cardiac function. Here we investigate miR-499, a microRNA embedded within a ventricular-specific myosin h...
متن کاملCoupling of mitochondrial function and skeletal muscle fiber type by a miR‐499/Fnip1/AMPK circuit
Upon adaption of skeletal muscle to physiological and pathophysiological stimuli, muscle fiber type and mitochondrial function are coordinately regulated. Recent studies have identified pathways involved in control of contractile proteins of oxidative-type fibers. However, the mechanism for coupling of mitochondrial function to the muscle contractile machinery during fiber type transition remai...
متن کاملMiR-499-5p protects cardiomyocytes against ischaemic injury via anti-apoptosis by targeting PDCD4
Recent studies have reported that miRNAs might play critical roles in acute myocardial infarction (AMI). The objective of this study is to investigate the role of miR-499-5p in AMI and its potential molecular mechanisms. The expression level of MiR-499-5p was remarkably decreased in the infarcted myocardial tissues and in the cultured neonatal rat cardiomyocytes induced by hypoxia. Overexpressi...
متن کاملMiR-499 Enhances the Cisplatin Sensitivity of Esophageal Carcinoma Cell Lines by Targeting DNA Polymerase β.
BACKGROUND Human DNA polymerase β (DNA polymerase β, polβ) is a small monomeric protein essential for short-patch base excision repair (BER). It plays an important role in regulating the sensitivity of tumor cells to chemotherapy. METHODS Luciferase reporter and western blot assays were used to determine whether polβ is a major target of miR-499. CCK-8, colony-forming survival and in vivo tum...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Circulation Research
سال: 2012
ISSN: 0009-7330,1524-4571
DOI: 10.1161/circresaha.111.260752